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Arichard:Protocols/MDA on microarray 2013 09 05
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====Loading==== * All samples should be suspended at the correct dilution in 3 ul media. ** Samples should also be stained at 1X with SYBR Green if the array loading is to be imaged prior to lysis. * Load each sample into its own array. Move tip back and forth across array while pipetting down, like a printer. Aim for uniform loading. Avoid the edges of the gasket. * Cover with coverslip and press down to seal against gasket. * To image array loading: Image under fluorescence (GFP channel), saving each image and position using multi-dimensional acquisition. Record 3 non-overlapping images per array. Cells will also settle during this time, ~10 min. ** Imaging is not necessary for chromosomal samples, as loading concentration will be high enough to assume every well contains template. Let chromosomal samples settle for 10 min.
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