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Blue:RNA-Seq Experiments:totoRNAseq RNaseII Test
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== Final Library Preparation == *Re-Amplified 1ul using Sybr-Green and ILMN_PCR_F/R ::{| {{table}} |- |||||||||||<u>Volume</u> |- |Library||||||||||1ul |- |2xSybr Buffer||||||||||25ul |- |10uM ILMN_PCR_F[http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNAseq_Primer_List#totoRNA_5.27_primer_sequence]||||||||||1ul |- |10uM ILMN_PCR_R[http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNAseq_Primer_List#totoRNA_3.27_primer_sequence]||||||||||1ul |- |dH2O||||||||||22ul |- |Total||||||||||50ul |- |} :::''95C 30sec'' :::''95C 10s -> 60C 20s -> 72C 30s'' => 5X :::''72C, 5min'' *Ampure Beads purified (0.8x) *Resuspended in 10ul and ran 1 ul on gel: [[File:08072013_PCR2_gel1.jpg]] Re-amplified 0.5ul library using un-modified ILMN_PCR_F/R and ran 5ul of 50 on gel:
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