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===Sample C=== Sample C is the dystrophin gene labeled with Alexa 488. The sample was not vacuum centrifuged, but rather 5 uL sample was added directly to the hybridization buffer. <gallery> File:20140806_DMD-488_Dilute_PreWash.lif_DMD488_D1_ch00.jpg|Cells stained with DMD, Alexa 488 (green), field 1 File:20140806_DMD-488_Dilute_PreWash.lif_DMD488_D2_ch00.jpg|Cells stained with DMD, Alexa 488 (green), field 2 </gallery> There is not strong signal shown in the majority of cells. This probably makes sense, however, since the concentration of probe is considerably less than the other samples. Post taking these images, I returned the samples to the incubator and set the incubator to '''37''' C, not 42 like I usually do. They will hybridize more overnight, and I will take more images [[Daniel:Notebook/HiResChrPaint/2014-8-7|tomorrow]]. [[Category:HRCP]] [[Category:Microscopy]] [[Category:20140805]]
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