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===Results=== Amplified 10ul capture mix with Amp6.3F/R NH2 primers B, C, D, E = 17 PCR cycles A, F-K = 15 PCR cycles [[File:MultivariateCaptureDMR220k-A.png]] [[File:MultivariateCaptureDMR220k-B.png]] '''Comparing JD vs KZ''': 97K control does not need the extra 2 cycles. PGP1-iPS capture seemed to require those two cycles because of low input concentration. => No noticeable differences between the two. '''97K probes set''': performs better at 58C than 60C (C vs E), (A vs K) '''220K probes set''': capture is more efficient at 58C than 60C (F,H vs I,J). Results from 200uM and 50uM vary. '''Low probes amount''': capture amplicons has more smearing for high probes input (176ng). See F (44ng DMR220K) vs G (176ng DMR220K) The following samples were purified and purified using 6% TBE gel for N2 adaptor protocol. B and D (PGP1-iPS) F,G, and H (PGP1-fib) [[File:PGP1-ips, PGP1-f 220k capture lib quant.jpg|400px]] 1. B-10.16ng/ulx20ul=203ng (PGP1-iPS captured at 58C, 44ng 220K probes (30:1), size selected) 3. D-12.76ng/ulx20ul=255ng (PGP1-iPS captured at 60C, 44ng 220K probes (30:1), size selected) 5. F-3.22ng/ulx20ul=64ng (PGP1-fib captured at 58C, 44ng 220K probes (30:1), size selected) 6. G-1.18ng/ulx20ul=24ng (PGP1-fib captured at 58C, 44ng 220K probes (30:1), size selected) 7. H-10.89ng/ulx20ul=327ng (PGP1-fib captured at 58C, 176ng 220K probes (100:1), size selected) 8. I-12.53ng/ulx30ul=376ng (PGP1-fib captured at 60C, 44ng 220K probes (30:1), non-ss) 9. J-4.28ng/ulx30ul=129ng (PGP1-fib captured at 60C, 44ng 220K probes (30:1), non-ss)
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