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== Agarose gel size selection == * Prepared >4% agarose gel (Apex multipurpose agarose). 1 g in 25 mL. UV for 15 minutes. * Put on heater/mixer at 200 degC let sit for about 5-10 minutes. Turn off the heat. * Fill a 2 L beaker partly with water and put the flast with melted agarose inside. Continue stirring for 10 minutes to cool the agarose solution. * Added 2uL of Sybr Gold per 25mL of agarose solution when the agarose solution have cooled enough to hold for 15 seconds. * Cast a gel of about 7 mm thickness with 3-5 mm wells. * Load at least 700 ng into each well. Purification will not work with less than 700 ng minimum input. * Ran at 135V for 45 minutes, the probes were in the bottom quarter of the gel by the end of electrophesis run. * Gel image: * After sample finished running. Weight a 50 mL tube. * Cut out 2 wells per tube and re-weigh to calculate 6X volume of QX1 buffer to add to each tube. * Add 10 uL of QIAX suspension solution to each tube. * Incubate at 60 degC for 6 minutes. Vortex every 2 minutes. * Label 2 mL tubes for spinning down the solution. Remove supernatant after each spin. * Wash with 500 uL QX buffer once. * Wash with 500 uL PE buffer three times. * Dry the pellet in the vacuum for 2 minutes. * Resuspend the pellet with water.
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