Editing
Hosuk:LabNotes/2013-11-6
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
====Result==== *ACTB only had more than 1st Rolony+ACTB *RAB7A only had more than 1st Rolony+RAB7A *ACTB+RAB7A was similar to ACTB only or RAB7A only. =====Summary===== {| {{table}} border = 1 | || | ACTB || | (%) || | RAB7A || | (%) |- | w/i 1st Rolony || | 74 [[Media:Exp2_DyeTest.lif_Step5_1stRolony_ACTB_20x_2k_Cy5_ATTO488_ch01_MIP.tif|Raw]] || | 1.45 || | 114 [[Media:Exp2_DyeTest.lif_Step3_1stRolony_RAB7A_20x_2k_Cy5_ATTO550_ch01_MIP.tif|Raw]] || | 2.25 |- | 1 probe only || | 157 [[Media:Exp2_DyeTest.lif_Step6_AddACTBOnly_20x_2k_Cy5_ATTO488_ch01_MIP.tif|Raw]] || | 3.08 || | 260 [[Media:Exp2_DyeTest.lif_Step4_AddRAB7AOnly_20x_2k_Cy5_ATTO550_ch01_MIP.tif|Raw]] || | 5.13 |- | ACTB+RAB7A || | 130 [[Media:Exp2_DyeTest.lif_Step7_AddACTB_RAB7A_20x_2k_ATTO488_550_ch00_MIP.tif|Raw]] || | 2.55 || | 268 [[Media:Exp2_DyeTest.lif_Step7_AddACTB_RAB7A_20x_2k_ATTO488_550_ch01_MIP.tif|Raw]] || | 5.25 |} *ACTB + RAB7A : Overlapped spots from each other ? **Overlapped Count of ACTB with RAB7A, Count = 26 --> Overlapped with 1st Rolony : 14 **Overlapped Count of RAB7A with ACTB, Count = 26 --> Overlapped with 1st Rolony : 15 **There are about 10% (ACTB) or 5% (RAB7A) overlapped counting, which is not small number...those spots need to be neglected. *ACTB on ACTB only and ACTB on 1st Rolony + ACTB, : Overlapped spots from each other ? **Overlapped Count of ACTB on ACTB only with ACTB on 1st Rolony , Count = 120 **Overlapped Count of upper result with 1st Rolony , Count = 70 --> similar to the number from ACTB with 1st Rolony, 74 *RAB7A on RAB7A only and RAB7A on 1st Rolony + RAB7A : Overlapped spots from each other ? **Overlapped Count of RAB7A on RAB7A only with RAB7A on 1st Rolony , Count = 584 **Overlapped Count of upper result with 1st Rolony , Count = 260 --> exactly same to the number from RAB7A Only =====Counts===== *Step1 : RAB7A only, **RAB7A, Count = 514, Overlapped = 160 *Step3 : 1st Rolony + RAB7A, **1st Rolony Count = 5066 **RAB7A, Count = 186, Overlapped = 114 *Step4 : RAB7A only **RAB7A, Count = 591, Overlapped = 260 *Step5 : 1st Rolony + ACTB **1st Rolony Count = 5105 **ACTB, Count = 137, Overlapped = 74 *Step6 : ACTB only **ACTB, Count = 246, Overlapped = 157 *Step7 : ACTB + RAB7A only **ACTB, Count = 234, Overlapped = 130 **RAB7A, Count = 463, Overlapped = 268 =====Pictures===== {| {{table}} | ACTB with 1st Rolony (Overlapped Count = 74) || | ACTB only (Overlapped Count = 157) |- | [[File:Exp2_DyeTest.lif_Step5_1stRolony_ACTB_20x_2k_Cy5_ATTO488_ch00_MIP.tif_composite2.png|500px]] || | [[File:Exp2_DyeTest.lif_Step5_1stRolony_ACTB_20x_2k_Cy5_ATTO488_ch01_MIP.tif_composite2.png|500px]] |} {| {{table}} | RAB7A with 1st Rolony (Overlapped Count = 114) || | RAB7A only (Overlapped Count = 260) |- | [[File:Exp2_DyeTest.lif_Step3_1stRolony_RAB7A_20x_2k_Cy5_ATTO550_ch00_MIP.tif_composite2.png|500px]] || | [[File:Exp2_DyeTest.lif_Step4_AddRAB7AOnly_20x_2k_Cy5_ATTO550_ch01_MIP.tif_composite2.png|500px]] |} {| {{table}} | ACTB on ACTB+RAB7A (Overlapped Count = 130) || | RAB7A on ACTB+RAB7A (Overlapped Count = 268) |- | [[File:Exp2_DyeTest.lif_Step7_AddACTB_RAB7A_20x_2k_ATTO488_550_ch00_MIP.tif_composite2.png|500px]] || | [[File:Exp2_DyeTest.lif_Step7_AddACTB_RAB7A_20x_2k_ATTO488_550_ch01_MIP.tif_composite2.png|500px]] |}
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information