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==PCR(09/01/2008)== x6 Template 10ul 2X iProof Mastermix 50ul 300ul AmpF6.2SoL (10uM) 4ul 24ul AmpR6.2SoL (10uM) 4ul 24ul 50X SYBG I 0.8ul 4.8ul H2O 31.2ul 187.2ul 98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 10-> 72C 5 min -> 15C hold. [[Image:20080901_cpg30kV2.2_captured PCR_No_1_2_3_9_Jurkat.jpg]]20080902_cpg30kV2.2_captured PCR_No1_2_3_9_Jurkat [[Image:20080901_Quantification of No1_2.jpg]]20080901_quantification of No_1_2 [[Image:20080901_quantification of No_3_9.jpg]]20080901_quantificatio of No3_9 yield: No_1: cpg30k + suppressor: 6ng/ul x 15ul V2.1: 6ng/ul x 15ul V2.2: 4ng/ul x 15ul V2.3: 6ng/ul x 15ul V2.2_09/01/08: 3ng/ul x 15ul mix 5ul cpg30k + suppressor + 5ul V2.1 + 10ul V2.2_09/01/08 + 5ul V2.3 = 4.8ng/ul x 25ul No_2: cpg30k + suppressor: 6ng/ul x 15ul V2.1: 6ng/ul x 15ul V2.2: 3ng/ul x 15ul V2.3: 5ng/ul x 15ul V2.2_09/01/08: 4ng/ul x 15ul mix 5ul cpg30k + suppressor + 5ul V2.1 + 7.5ul V2.2_09/01/08 + 6ul V2.3 = 5ng/ul x 23.5ul No_3: cpg30k + suppressor: 4.5ng/ul x 15ul V2.1: 4.5ng/ul x 15ul V2.2: 2.5ng/ul x 15ul V2.3: 2.5ng/ul x 15ul V2.2_09/01/08: 2ng/ul x 15ul mix 5ul cpg30k + suppressor + 5ul V2.1 + 4ul V2.2 + 9ulV2.3 + 5ul V2.2_09/01/08 = 3.2ng/ul x 28ul No_9: cpg30k + suppressor: 5ng/ul x 15ul V2.1: 3ng/ul x 15ul V2.2: 1.5ng/ul x 15ul V2.3: 2ng/ul x 15ul V2.2_09/01/08: 3ng/ul x 15ul mix 4ul cpg30k + suppressor + 7ul V2.1 + 10ul V2.3 + 7ul V2.2_09/01/08 = 2.8ng/ul x 28ul
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