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===2nd PCR amplification with AmpF6.3/AmpR6.3 and dUTP:dNTP 1:40 === reaction system x14 x2 H2O 42.6ul 596.4ul 2x Master mix 50ul 700ul dUTP(1mM) 2ul 28ul AmpF6.3(10uM) 2ul 28ul AmpR6.3(10uM) 2ul 28ul 50x SYBG I 0.4ul 5.6ul template(4ng/ul) 0.5ul 2 uleach Total 100ul 1400ul 94C 3min -> 9cycles of (94C 45sec -> 55C 45sec -> 72C 45sec) -> 72C 3min -> 4C Bioneer column purification. Yield: No_21:40.5ng/ul; No_22:39.1ng/ul; No_23:35.6ng/ul; No_24:32.4ng/ul; No_25:47ng/ul; No_26:35.2ng/ul; No_21_2: 28.9ng/ul;
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