Editing
Jie:LabNotes/CpgSeq/2009-5-20
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
===PCR with AmpF/R6.3Sol and iProof=== x2 Template 5ul 2X iProof Mastermix 50ul 100ul AmpF6.3SoL (10uM) 4ul 8ul AmpR6.3SoL (10uM) 4ul 8ul 50X SYBG I 0.8ul 1.6ul H2O 40.2ul 80.4ul 98C 30S -> (98C 10S -> 72C 20S) x 11 -> 72C 3 min -> 15C hold. After Qiaquick purification, use the nanodrop to calculate the concentration: No09_1: 19.3ng/ul (73nM) x 30ul; No09_7: 23.1ng/ul (87nM) x 30ul. [[Image:20090526_amplicons after normalization of captured targets.jpg]]20090526_amplicons after normalization of captured targets I sent 10ul of the 09_7 NA21070*1 100406 captured targets and 09_7 NA21070*1 100406 normalized captured targets to do the end sequencing and see the read abundance of probes. The sample names are labeled as: 21070*1 100406 captured targets: 96nM ; 21070*1 100406 normalized : 87nM.
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information