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====PCR amplification with AmpF6.3NH2/AmpR6.3NH2 (2009-07-14)==== I did the PCR reamplification with Amp6.3NH2 primers. 400ul for each sample. reaction system x4 x10 H2O 45ul 180ul 2x Mastermix 50ul 200ul AmpF6.3NH2(10uM) 2ul 8ul AmpR6.3NH2(10uM) 2ul 8ul 50x SYBG I 0.4ul 1.6ul template 1ul/each for purified cpg97k Total 100ul 800ul 94C 2min -> (94C 45S -> 55C 45S -> 72C 45S) x 8 ->72C 3 min -> 15C hold. [[Image:20090714_Parkinson samples with AmpF6.3NH2 reamplification.jpg]]20090714_Parkinson samples with AmpF6.3NH2 reamplification I purified the samples with Qiaquick column. Quantify with nanodrop and send the samples to Billy.
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