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===redo the ASE PCR=== No74: CPNE8 G1 62C annealing 45cycles GLANT9 H1 58C MATN4 D3 62C GLIPR1L2 B3 62C No65: GLANT9 H1 MATN4 D3 GLIPR1L2 B3 62C No75: GLANT9 H1 MATN4 D3 GLIPR1L2 B3 62C No64: GGT7 A3 62C GLIPR1L2 B3 64C C20orf85 B1 58C No72: C20orf85 B1 No73: C20orf85 B1 [[Image:20090711_ASE_redo.jpg]]20090711_ASE_redo I sent the 22 samples to do the sanger sequencing on 09-07-13. [[Media:20090713_plate_to_genewiz_ASE_valid.xls|20090713_plate_to_genewiz_ASE_valid]] I got the results validated the monoallele expression of GLIPR1L2 of GM10864. I got the results validated the monoallelic expression of GLIPR1L2 on GM10865, 74 and 75 family. <BR>However, this gene is not imprinted gene. It demonstrated ASE. I designed the primer to validate the ASM of GLIRP1L2. [[Media:20090719_primer_GLIPR1L2_ASM_valid.xls|20090719_primer_GLIPR1L2_ASM_valid]] [[Media:20090719_GLIPR1L2_ASM_gDNA_target_seq.txt|20090719_GLIPR1L2_ASM_gDNA_target_seq]] I did not get the specific amplification of MATN4 and GLANT9.
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