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==Data analysis== *The first four libraries were sequenced on 03/07/09. Library #raw reads #mappable reads #targets PGP1AC 8,948,696 147,867/152,555 7,593,058 PGP1AC-Norm1 9,933,208 143,790/152,555 8,016,337 PGP1AC-Norm2 5,008,442 139,647/152,555 4,143,134 PGP1S 7,974,771 145,500/152,555 6,592,144 Representation bias [[Image:2009-03-07-Exome-Jan09_set-representation-bias.png]] *Summary **The four libraries have quite similar bias, and the distribution of bias is quite similar to the hybrid capture method published in the Nature Biotech paper. **Post-capturing normalization didn't seem to work. Three possibilities: ***The ds-DNA nuclease lost its activity; ***The complexity of the library is >10x higher than the Oct08 set. ***The library was not biased enough to be normalized. Perhaps it takes longer for the DNA to anneal. ===Histogram of target counts=== {| {{table}} | [[Image:2009-03-07-Exome-Jan09-PGP1AC-dist.png|300px]]||[[Image:2009-03-07-Exome-Jan09-PGP1S-dist.png|300px]] |- | [[Image:2009-03-07-Exome-Jan09-PGP1AC-norm1-dist.png|300px]]||[[Image:2009-03-07-Exome-Jan09-PGP1AC-norm2-dist.png|300px]] |- | |}
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