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==== Adapter preparation ==== Stoffel buffer 6ul PE_t_adapter 2ul PE_b_adapter 2ul -------------------------------------- Total 10ul 94C 2min -> 0.2C/sec to 20C -> 4C hold '''x8.2 mix for Gr.1''' '''x5.2 mix for Gr.2''' A-tailed DNA 35ul - - Adapters 1ul 8.2ul 5.2ul (Adapter was diluted 10x in Stoffel buffer) QuickLigase 2ul 16.4 0.4 2xQuickLigase Buffer 38ul 312 198 -------------------------------------------------------------------------------------- Total 76ul - aliquot 41ul into each tube & add 35ul of A-tailed DNA - Incubate at RT fo 15 min. Purify with 0.7x AMPure beads. Elute with 40ul EB.
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