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====Imaging==== #Add 200ul of 0.5uM FISSEQ_Adpt Cy3 in 2X SSC + 30% formamide pre-heated to 75C #Incubate for 10min at RT #Wash with 2X SSC twice #Added 200ul 5uM DRAQ5 (diluted in PBS from 100uM brown tube Hosuk made) to tissue and incubated 15min at RT *552 Laser 1% Cy3 Gain 550 *638 Laser 5% Gain 800 *Images not shown here because they aren't as good as the images from 2nd session **The first z-slice starts in middle of embryo so misses some sections of embryo nearest to the glass #Replace buffer with 1X PBS and stored in 4C over the weekend
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