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==Results== [[Media:20170423_qPCR_PPcapture_SplintRformamideETSSB.xlsx|raw data here]]<br> [[File:20170423_qPCR_ppCapture_SplintRformamideETSSB.PNG|650px]] *Possible options: 10% formamide [[File:20170423_qPCR_PPcapture_SplintRformamideETSSB_ScatterPlot.PNG|450px]] *No difference between 15min and 30min SplintR incubation times *ET SSB added does increase the number of captured padlock probes **Adding ET SSB before SplintR vs during SplintR makes no difference *Next time do a ET SSB + 10% formamide sample *Next time try less template concentration... see if it can distinguish the smaller amount
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