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==Repeat qPCR== #qPCR all 16 samples 1ul each with triplicates {| {{table}} | align="center" style="background:#f0f0f0;"|'''Components''' | align="center" style="background:#f0f0f0;"|'''1X Volume''' | align="center" style="background:#f0f0f0;"|'''48X Volume''' |- | Captured template||1||0 |- | 10uM ISB_CA_AF||0.4||19.2 |- | 10uM ISB_CA_AR.T2||0.4||19.2 |- | 2X KAPA SYBG MM||12.5||600 |- | H2O||10.7||513.6 |- | Total||25||1,152 |} *Aliquot 24ul from 48X master mix and add 1ul captured template Program 98C 1min -> (98C 10s -> 52C 20s -> 72C 20s)x30 -> 72C 3min [[File:20170515_qPCR_PPcapture_SplintRformamideETSSB_triplicates_ScatterPlot.PNG|450px]] *Full analysis here: [[Media:20170515_qPCR_PPcapture_SplintRformamideETSSB.xlsx]]
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