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== CAUSE OF PROBLEM == * I never used this eppendorf plate for bisulfite conversion before. It should be the sealing problem because the well beside without DNA were sealed with plastic seal whereas the well with reaction sealed with a smalled strip of aluminum seal. The partial overlapping between the aluminum seal and plastic seal (on bottom) cause the reaction leaking. In the future, I should pay attention on sealing the plate seriously, since this problem never happen when I used the plate came with the kit and I did the bisulfite conversion on the whole plate and seal very well. * After I tested the plated by incubating few well of reagent at the same thermalcycler profile for Lightning MagPrep, I found that it's possible that I forgot to set the machine to heat lid. This because i observed the water drop on the top of the well even it didn't leak out like last time, but I didn't observed this when I heated the lid. * Continued on: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2012-8-1
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