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===Dr. Yang Xu lab=== * 6/27/11: # GFP(+), Pancreatic lineage differentiated from hESC, NGN3+ (~50000 cells, purified with Qiagen RNeasy), 20.8ug/ml, use 1ul # GFP(-), Pancreatic lineage differentiated from hESC, NGN3- (~50000 cells, purified with Qiagen RNeasy), 12.7ug/ml, use 1ul cDNA library lab note: [http://genome-tech.ucsd.edu/LabNotes/index.php/Kun:LabNotes/SingleCellExpr/2011-7-5] RNAseq library lab note: [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/Collaborations/2011-7-11] RNAseq run analysis: [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui_Liu#HL098] * 7/7/11: Origin (?) Method (cell sorting --> spin top speed for at least 5min, done by Dae-Hwan) # Negative control (No fluorescence)--- 2 tubes (>200,000 cells each) # RFP positive ------ 1 tube (~12,000 cells) # GFP/RFP double positive ------ 1 tube (~55,000 cells) cDNA library lab note:[http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/Collaborations/2011-10-3#cDNA_libraries] RNAseq library lab note: [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/Collaborations/2011-10-11] RNAseq run analysis: [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui_Liu#HL106] * 9/28/11: 3 samples from Dr. Xu lab by Alice, RNA samples from sorted neural stem cells from nestin-rfp knockin hESCs. # Low is rfp low # high rfp high # fetal NSCs are the human fetal cells. cDNA library lab note:[http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:LabNotes/Collaborations/2011-10-3#cDNA_libraries] RNAseq library lab note: RNAseq run analysis:
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