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==Test of loading cell on PDMS microwell (provided by Jeff)== *Newly fabricated PDMS microwell (id: 400um) appears to be an ideal sample reservoir for the micro-manipulation. *The smaller volume should help to restrain the floating of E.coli cell in suspension, which is beneficial to target capturing. *Possible isolation approach: #Dilute the E.coli cells into desired density. #Transfer E.coli cells into microwell. #Identify the wells containing only single E.coli cell. #2nd transferring from microwell to PCR tubes. ===Procedures=== *Since the PDMS microwell is very thick, it should be put on another station for microscopy. *Using 4X objectives for observation. ===Results=== [[File:Jeff-PDMS-microwell-BF-4X-new.png|400px]] [[File:Jeff-PDMS-microwell-DAPI-4X-new.png|400px]] [[File:Jeff-PDMS-microwell-DAPI-4X-washed2-new2.png|400px]] ===Discussion=== *Compared to SU-8 based microwells, PDMS-based microwells has less autofluorescence problem and can serve as good sample reservoirs. *After washing microwell using 100% isopropanol, I remove most of dusts but the well are still not clean enough for microisolation.
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