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===Follow up: AMpure bead purification (11-19-'10)=== *The library pass the Sanger sequencing validation. 14/16 sequences results showed E.coli positive. **http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-16_exp1 *Since the library still carry short(~50bp) fragments (shown on PAGE), I did an additional AMpure bead purification to remove these short fragments: **Starting amount is set up as 30uL, I use 1V of AMpure beads(30uL) to bind on library DNA. **Wash with 200uL 70% EtOH twice, air dry 2min. **Elute DNA in 20uL pre-warmed H2O. *Library was submitted to Alan for sequencing. Library tag: '''HC-MDA-Ecoli-SAGs(Ind1to8)-Nov15'''
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