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=== Step 3. Prepare the bisulfite-converted DNA sample library and HE oligos for hybridization === # Have ~1ug of the WGBS library pool in a Lo-bind 1.5 mL tube prepare above, including normal control WGBS library pool and cancer patient WGBS library pool # Thaw the resuspended SeqCap HE Universal Oligos (1000uM) and the resuspended SeqCap HE Index Oligo (1000uM) that matches the DNA adaptor index in the WGBS library preparation ::- In this experiment, I pooled all libraries using barcode adaptor match with all 24 indexes in HE-Oligo KitA/B (0.8ul each). I pool the same amount of the 24 indexes in kit A & B and use 1ul of the pooled oligos in step 4.3
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