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==post-hybridization amplification and purification== *Prepare the Post-Capture PCR Master Mix in a 1.5ml tube. *Aliquot 5μl of bead-bound captured DNA as template into 3 PCR tubes/wells. Mix well by pipetting up and down 5 times. x3 post-capture DNA: 5ul Phusion High-Fidelity PCR Master Mix (2x): 50μl PCR grade water: 41ul Syb_FP5, 100 μM (Final Conc.: 2μM): 2ul Syb_RP7, 100 μM (Final Conc.: 2μM): 2ul SYBR green 50x: 0.4ul Total : 100ul 98C 30sec -> (98C 10sec -> 60C 30sec -> 72C 30sec) x 13 cycles -> 72C 5min -> hold at 4C *Qiaquick column purification and elute in 40ul EB
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