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=== SLN mix === {| {{table}} border = 1 | align="center" style="background:#f0f0f0;"|'''Components''' | align="center" style="background:#f0f0f0;"|'''Stock conc.''' | align="center" style="background:#f0f0f0;"|'''Unit''' | align="center" style="background:#f0f0f0;"|'''Final conc.''' | align="center" style="background:#f0f0f0;"|'''Unit''' | align="center" style="background:#f0f0f0;"|'''Prepare volume 150''' |- | AmpliTaq Stoffel||10.00||U/ul||2.00||U/ul||30.00 |- | AmpLigase||5.00||U/ul||0.50||U/ul||15.00 |- | dNTP||1.00||mM||100.00||uM||15.00 |- | 10x AmpLigase Buffer||10.00||x||1.00||x||15.00 |- | H2O||||||||||75.00 |- | ||||||||Total||150.00 |}<br> * aliquot 15ul of SLN to strip tube, and add 2.5 ul to each tube. * Note: ** There was something wrong with sample in well A9-A12. The volume of reaction in these four well was lower than 10ul, but before capture the levels of the reaction in every well were the same. I think that it's possible that the 96-well plate was not sealed very well especially the border of the plate, so the evaporation possibly occurred. There was proof that the reagents were not missing added to these four wells since one of them could be amplified, but not in a good yield. In the future, there should be more concerns about the plate sealing. ** It would be better if the samples will be rearranged from A1-H1, ..., A2-H2,.... rather than A1-A12, B1-B12. This because the multichannel pipette has 8 channels, so it would be easier to add samples in the same column at once. In addition, it's less possibility to miss adding reagents in well No.9-12<br>
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