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== PAGE verification == [[File:ZhangLab_2 2013-03-07 17hr 02min_TestExpansionPCR_variedconc.jpg| 300px]] O = 2ul of 12k oligos from original tubes * Note: I loaded 2.5ul of PCR products in TBU gel, so the size might be slightly different compared to in TBE gel. However, from the gel image, the size of amplicons is very close to 200bp fragment. I loaded PCR products in TBU gel becuase I didn't want to waste the TBU gel used for single strand oligos verification * From the image above, the amount of oligos in 2ul from original tubes was very high, but they all a smear. This is similar to what I observed when I ran the oligos from LC Sciences. * Since I used very high concentration of oligo templates, the smear DNA were observed. * For expansion PCR, I may not amplify as high as 20 cycles as in the test run. Need to decide the amount of oligos will be used for expansion PCR if I will amplify 15 cycles. <br> === 2013_03_08 === * Dr. Zhang suggested to do expansion PCR using 100nM oligos
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