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=== Experimental plan II === * I will do BSPP capture by varying bisulfite converted gDNA amount to see the minimum amount of 12K BSPP probe set that work well for capture experiment. * I will also vary the probe: target ratio. As I know that the DMR330k BSPP probe set works well with the ratio ~100:1 - ~200:1, but I have adjusted the probe:target ratio in each set. In addition the DMR330K are muck more larger than the BSPP_Blueprint probe set. I may need to increase the probe:target ratio. * I will set up BSPP capture with template amount 50ng, 100ng, 150ng and 300ng (200-300ng is the amount that I usually used and worked well for previous experiment) * Each template amount will be captured with 200:1 and 500:1 probe:target. I actually should have done 1000:1 for comparison too.
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