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==TBU-PAGE size selection== *A 5-well TBU gel is recommanded since it has large capacity for sample loading. **Sample: 10uL of ligation reaction + 10uL 2X TBU-loading dye **Ladder: 0.5uL 25bp ladder + 9uL H2O + 10uL 2X TBU-loading dye **Heat denaturing: 75C 15min -> 1min on cool block **Flush TBU gel well. Pre-run 10min at 200V **Flush TBU gel well again. Load denatured samples and ladders(20uL). Run at 200V for 30min. **Stained with 3uL SYBR-Gold in 0.5X TBE for 5min [[Image:sam102609-TBU-4th test.jpg|450px]] [[Image:sam102609-TBU-4th test -post cut.jpg|450px]] Left: Pre-cutting Right: Post-cutting NOTE1: I miscalculate the band size of L5 and L6 samples in the beginning, but I added those part by additional cutting. NOTE2: The gel seems overloading. It is suggested that no more than 100ng/well should be loaded in 12-well PAGE.
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