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==Results== *Samples were picked from Realplex machine when they arrived about 10000~15000 increasement of fluorescence. [[File:sam122910-qPCR-1.jpg|900px]] '''Monitored fluorescence''' [[File:sam122910-qPCR-3.jpg|900px]] '''Normalized fluorescence''' Sample NX1 NX2 NX3 NX4 ---------------------- Well A1 A3 B2 B4 Min 33 29 58 29 Cycle 7 6 13 6 Dif. Fluorescence(monitored) 12000 8000 11000 8000 *'''The starting fluorescent intensities for each of templates are consistent with their estimated DNA concentration (by QUBIT-HS)''', which suggested that the QUBIT-HS quantification is relatively accurate. Library DNA starting amount qPCR starting fluorescence(monitored) NX1 288ng 10000 NX2 500ng 17000 NX3 114ng 4000 NX4 500ng 16000
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