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AlanFung:Protocol/Genome Analyzer Reagents Prep
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==Cleavage Mix== Required Materials Cleavage Reagent (5ml) Cleavage Mix Diluent (40ml) Cleavage Mix Additive 10X Cleavage buffer v2.0 (5ml left over from 1X cleavage Buffer preparation) Plastic Syringe 0.22um Cellulose Acetate Syringe Filter 50ml Tube (User-Supplied) *Place the Cleavage Reagent in a 37°C circulating water bath. *When the reagent is completely thawed, invert it several times to mix *Transfer it back to ice immediately *Add 5 ml of 10X Cleavage Buffer v2.0 to the tube of Cleavage Mix Diluent *Add 5 ml of the thawed Cleavage Reagent to the tube of Cleavage Mix Diluent *Invert several times to mix. *Add the Cleavage Mix Additive to the Cleavage Mix Diluent tube. This solution is now called the Cleavage Mix. Rinse the empty additive tube into the Cleavage Mix three times with 1 ml of Cleavage Mix. *Invert several times to mix. Return the tube to ice and leave it until the solids are completely dissolved *Filter the Cleavage Mix into a new 50 ml tube using the syringe and 0.22 μm cellulose acetate syringe filter provided in the kit *Label the tube “Cleavage Mix.” *Record the weight of the reagent in the lab tracking worksheet *Return the filtered mix to ice immediately *Discard your gloves and replace with a new pair
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