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===March 07=== * Started new run. Will start IVT tonight, mg frag tomorrow morning followed by PAGE gel. * Same setup as before, except for two additional samples (positive and negative controls). * Positive control is half volume relative to the Ambion manual, and 5X volume relative to CEL-Seq. Both have 0.5 ul of the T7 primer that was shipped with the kit (unknown concentration). The negative control has 5 ul of UV treated H2O, while the positive control has 5 ul of 100 ng/ul total RNA (from Rui). These two samples will not undergo the Bst reaction to add the T7 primers, since they already have T7 promoter sequences.
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