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===Size selection=== - Select 450bp band or 400 - 600bp band. - Perform PAGE size selection with 6% TBE 2D gel by cutting the band in the range of 400 - 600bp (x4 tubes, dissolved with 11ul EB, total vol. 44ul ). [[File:ZhangLab_2 2010-10-27 13hr 08min_MG SAGs SeqLib SS PAGE.jpg|300px|Image: 300 pixels]]<br> Figure6, MG SAGs BatchA Sequencing Library PAGE size selection (25bp ladder) - PAGE analysis to check the size and conc. of DNA library. - From the gel, the size of DNA range was too big (approx. 400 almost 800bp). The reason was I used 25 bp as the ladder, so I's hard to identify the band above 500bp (couldn't find 100bp ladder). -Dr. Zhang recommended Dinh to cut DNA in the range of 450 - 550 bp. - Perform PAGE size selection again with 6% TBE 5-well gel, eluted with 12ul EB. [[File:ZhangLab_2 2010-10-27 13hr 08min_MG SAGs SeqLib SS PAGE-round2.jpg|300px|Image: 300 pixels]]<BR> Figure7. MG SAGs BatchA Sequencing Library PAGE size selection (round2) 25bp ladder, 1kb ladder, 1st round PAGE SS purified sequencing library, 50bp ladder - 1ul of DNA was analyzed in PAGE and quantify DNA conc. = 13.8ng/ul or nM [[File:ZhangLab_2 2010-10-28 22hr 59min_MG SAGs-SSPAGE-final.jpg|250px|Image: 250 pixels]]<br> Figure8. LM 6ul, LM3ul, 2 round PAGE SS purified sequencing library, 1kb ladder, 25bp ladder
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