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===Nextera XT=== *Repeat with Derek's/Blue's reagents and protocol *Input ~0.2ng DNA *PosCtrl: 0.253ng/ul (diluted 1:10) *Exp: 0.226ng/ul (diluted 1:2) *NegCtrl: H2O *GM12878 gDNA: 0.201ng/ul (diluated 0.803 ng/ul 1:4) #Add 2.5ul Tagment DNA Buffer #Add 1.25ul Amplification Tagment Mix #Add 1.25ul Sample #Vortex and spin down #Incubate 55C for 10min and then hold at 10C #Add 1.25ul NT buffer and incubate at RT for >5min #Add 3.75ul NPM, 1.25ul Index Primer 1 and 1.25ul Index Primer 2 (S517) #*PosCtrl: N701 #*Exp: N702 #*NegCtrl: N703 #*gDNA: N704 #Vortex and spin down #Incubate in thermocycler: ##72C for 3min ##95C for 30sec ##12 cycles of: ###95C for 10sec ###55C for 30sec ###72C for 60sec ##72C for 5min
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