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== Capture plan == * Capture volume --> in 15-20ul * Vary annealing temperature --> try all below 55C we are currently using for DMR330k BSPP capture (since the probe size is shorter) --> 48C, 50, 52C, 55C '''Program'''<br> 95c 30sec -> cool down to 48-54 C at 0.02C/sec -> X C 17.5h<br> -> add 2ul SLN mix(2U/ul AmpliTaq Stoffel fragment; 0.5U/ul AmpLigase; 100uM dNTP)<br> -> 48-54 C 17h-> 94C 2min -> add 2ul Exo I/III mix-> 37C 2h -> 94C 2min -> 4C hold.<br> * Note: I try not to change the capture condition this time since want to make sure that everything will work well as in the past except varying the annealing temp.
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