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==== <u>1X AMPure bead purification</u> ==== * Actual protocol uses 0.8X AMPure beads '''''<span style="color:crimson"><u>Prep</u></span>'''''<br> - Freshly prepare 5mL of 80% EtOH by mixing 4mL of 100% EtOH with 1mL of H2O<br> - Aliquot 280uL of resuspened AMPure bead in 1.5mL tube :- Add 55ul AMPure bead. Mix by pipetting 10x :- Sit for 15min :- Transfer to sit on magnet for 5min :- Wash twice with 200ul freshly prepared 80% EtOH :- Dry the bead for 3-5min :- Resuspend with 21ul EB buffer :- Continue to bisulfite conversion immediately * Note: I paused at this step by putting adaptor-logated DNA on ice ~3h to wait for the reactiosn from NEXTflex cell free DNA Seq protocol to continue to bisulfite conversion together. <br>
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