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===Addition of bPCR-Compatible Sites and Library Enrichment=== *1. Assemble the following reaction components at room temperature 10ul bilsufite converted library 25ul 2X Nextera PCR Buffer 1ul 50X Nextera Primer Cocktail 1ul 50X Nextera Adaptor 2 1ul Nextera MEthyl-Seq Polymerase *2. Cycle the samples in a thermocycler under the following conditions 95C for 30sec 25cycles of 95C for 10sec 60C for 10sec 72C for 1min hold for 1min [[File:3_30_2011.JPG|500px]] *3. Purify the tagged DNA using qiaquick column *Page Quantification [[File:2011-03-30 NTC.jpg|500px]]
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