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====Strip off and Re-hybridize with ppMALAT1==== #Strip all 3 mattek dish w preheated (75C) 80% formamide for 15 mins at RT #Wash 2X with 2X SSC #Add 200ul 2uM ppMALAT1 (100ul more than usual to avoid evaporation, also wrap dish with parafilm) in 2X SSC @45C for 1hour, 4 hour and 24 hours starts at 11:05am #Wash 2X with 2X SSC #Prepare dye for 3 scans (30ul 20X SSC, 90ul 100% formamide, 177ul H2O, 3ul Dye) #Preheat dye at 75C for 5 mins #Add 100ul 1uM dcProbe2RevComp-Cy3 in 2X SSC + 30% formamide #Incubate at RT for 10 mins #Wash w 1X PBS twice #Imaging
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