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===perform linearization blocking and primer hybridization=== *load reagents in the following positions as indicated *single-read recipe-load position 3,4,7,12,15,17 *paired-end recipe-load position 3,4,7,12,14,17 *click ok to resume the recipe and follow the prompt to proceed to linearization blocking and primer hybridization *load flow cell and attach the amplification manifold *click ok to proceed and follow the prompt to check for proper flow *as the process starts check for correct fluid flow through all eight lanes and the lines of the amplification manifold when the flow is regular in all lanes proceed with the protocol *click ok to proceed *wait for the message to let you know that the flow cell is ready for sequencing *remove the flow cell from the cluster station DO NOT STORE THE FLOW CELL AT THIS POINT FOR LONG PERIODS OF TIME. SEQUENCING MUST BE PERFORMED ON THE FLOW CELL WITHIN 4 HOURS *Measure the reagent volumes and record them on the lab tracking form. compare to the table provided
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