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==Step 5:Column purification with Qiaquick== Purify the reaction with Qiaquick columns * Add 5X sample volume of PB buffer into each tube, mix well * Load mixture onto column * Centrifuge at 14,000rpm for 1min * Discard flow through and add 750uL PE buffer to each column * Repeat Centrifuge * Discard flow through * Repeat centrifuge * Transfer columns to clean 1.5mL tubes * Place the column/tube assemblies on bench top wait for 2min * Add in 50uL EB buffer to each column (For nicking enzyme probes add in 100ul of ddH2O) let stand for 5 min * Repeat centrifuge * Measure concentration with Nanodrop
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