Editing
Alice:Exome/2012-5-24
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
==post-hybridization amplification and purification== *Prepare the Post-Capture PCR Master Mix in a 1.5ml tube. *Aliquot 20μl of captured DNA as template into one PCR tubes/wells. Mix well by pipetting up and down 5 times. 2 well for each sample set post-capture DNA: 20ul Phusion High-Fidelity PCR Master Mix (2x): 50μl PCR grade water: 26ul Syb_FP5, 100 μM (Final Conc.: 2μM): 2ul Syb_RP7, 100 μM (Final Conc.: 2μM): 2ul Total : 100ul 98C 30sec -> (98C 10sec -> 60C 30sec -> 72C 30sec) x 13 cycles -> 72C 5min -> hold at 4C Qiaquick column purification and elute in 50ul ddH2O total per sample *PAGE validation: [[File:ZhangLab_2 2012-05-21 21hr 21min.jpg]] [[File:ZhangLab_2 2012-05-24 09hr 04min.jpg]] [[File:ZhangLab_2 2012-05-24 09hr 03min-B3-hues9.jpg]]
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information