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===May 29, 2013=== * Single cell isolations on lymphocytes: ** Spent 2.5 hours this morning to get 2 cells, the second of which is questionable. ** First hour was spent getting the right cell dilutions, ETDA concentration, and camera settings. [[File: 2013_05_29_lymphocyte1.tif|400px]] Lymphocyte #1 [[File: 2013_05_29_lymphocyte2.tif|400px]] Lymphocyte #2 ** Will continue tomorrow. I need 7 or 8 single cells, plus one pool of ~10 cells. ** Later this week I will make libraries for these samples. * Today after lab meeting I will continue working on a transcript matrix for the single cell SMART-Seq data. So far I have about 8 datasets, but I need a faster way of collecting the information. * Once I have the data extracted from the files on genome miner, I will do PCA.
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