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=== Lab meeting discussion === * better terminology OR define terminology used better: polymorhophism, locus.. * obtain a p-value for assay to determine cancer or not cancer: First, determine what is the effect size we need? We need to be able to detect at least 0.1% difference. Next, we need to calculate the power and sample size needed to be able to detect at least 0.1% difference. Then sample that many cancer patient blood to create a distribution. * improve detection limit. Instead of using Chi-square, we can select locus by brute force : force all locus considered to be where 100% of cancer alleles are not observed in blood. * sample larger number of reads (all of the reads?) or many more times to generate a confident interval. * look for bisulfite sequencing data for blood/tumor online: can we use these data to create our distribution estimating a p-value? * test 5,6,7 cg locus.
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