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===Conclusion=== *Exonuclease digestion did not seem complete **Possibly adding 2uM of ppMALAT1 is too much for Exo I/III to handle in 2hrs? but doubt it **Use [[Matt:LabNotes/2014-4-6 | 10nM ppMALAT1]] next time to match the Exo I/III test *Because -Ampligase wells with +Exo still detected signal, cannot draw any conclusions about +Ampligase wells *The average % of detected rolonies of +Ampligase wells is less than average of -Ampligase wells: **Does Ampligase decrease the efficiency % of hybridization? **Or is it because the +Ampligase wells had more rolonies to start with? ***If that were true, it would imply ppMALAT1 is limiting reagent, which is unlikely given 2uM **Try adding 50% glycerol in -Ampligase wells next time to better mimic +Ampligase wells
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