Editing
Matt:LabNotes/2015-3-31
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
====Size Selection==== *5-well TBE Gel 220V for 32min **Low mass ladder **3 lanes of Exp **1 lane of NegCtrl *Cut out the bright band at 225bp [[File:2015-04-02_SeqRolony_SizeSelect.jpg|650px]] *Add to 2 0.5mls tube (with hole at bottom) in 2ml tubes **Spin at 15,000rpm for 3 min *Add 450ul 1X TE Buffer to each and shake at 37C for 1 hour *Filter out gel with 2 Nanosep columns **Spin at 15,000rpm for 3 min **Transfer solution, spin, then transfer gel and spin *Add 1000ul 100% EtOH, 45ul 3M NaOAc, and 1.4ul glycoblue **Vortex *Put in -80C freezer for 30min *Spun at 10,000rpm at 4C for 30min *Discard supernatant and add 650ul cold 80% EtOH and spin at 14,000rpm at 4C for 5min *Discard supernatant and let dry in hood for 10min *Resuspend each tube with 15ul and combine *'''FINAL:''' 30ul in 1.5ml tube labeled "MC-SeqRolony_BA8V4_Mar3115-13" and put in Non-quantified New Sequencing Libraries box with SeqRolony_RP1 and SeqRolony_IRP
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information