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====Zeiss Microscope in Huang Lab with Laser excitation==== *7/21/2015 *With help of Matt Walsh *Laser wavelengths: **488 **552? **642 *Exposure time: 100ms *Sample 1 - 488 (KIT) **Nothing to show, probably because gene expression is too low *Sample 1 - 594 (CUX2) **See combination of bright regions (guess is due to uncoupled dyes) and very few bright spots [[File:20150720_RNAFISH1_594.jpg]] *Sample 2 - 488 (SNAP25) **Lots of spots but also high background ***Hard to say significantly more than what I have seen with CUX2 (though based on DARTFISH and FPKM it should be) [[File:20150720_RNAFISH2_488.jpg]] *Sample 2 - 647 (CUX2) **Image is very grainy making it hard to tell distinct spots from noise, probably needs better excitation parameters [[File:20150720_RNAFISH2_647.jpg]]
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