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== ssDNA Qubit quantification == * Used 3ul of bis-cvt DNA for the assay (dilution 200/3) {| {{table}} | align="center" style="background:#f0f0f0;"|'''Sample''' | align="center" style="background:#f0f0f0;"|'''Concentration in the Qubit''' | align="center" style="background:#f0f0f0;"|'''Unit''' | align="center" style="background:#f0f0f0;"|'''uL used''' | align="center" style="background:#f0f0f0;"|'''Dilution''' | align="center" style="background:#f0f0f0;"|'''Final conc. (ng/ul)''' | align="center" style="background:#f0f0f0;"|'''Yields in 40ul''' |- | #1||17.2||ng/mL||3||66.67||1.15||45.87 |- | #2||4.4||ng/mL||3||66.67||0.29||11.73** |- | #3||12.3||ng/mL||3||66.67||0.82||32.80 |- | #4||65.6||ng/mL||3||66.67||4.37||174.93 |- | #5||32.9||ng/mL||3||66.67||2.19||87.73 |- | #6||7.16||ng/mL||3||66.67||0.48||19.09** |- | #7||48.3||ng/mL||3||66.67||3.22||128.80 |- | #8||30.6||ng/mL||3||66.67||2.04||81.60 |} * The yield after bisulfite conversion is relatively low when compare to initial amount of DNA. However, the DNA amount would be reduced in each step. From the image, bead purification did'n recover the fragments < ~200bp, so I don't expect to get very high after bisulfite conversion. The total yields I got after bisulfite conversion are varied as well, and the yield of some sample didn't correlated with the intensity before bisulfite conversion. However, I could not rely on to this image because it is the image of previous experiment. Next time I should collect sample for verification. * **= samples have very low yields --> If compare to the intensity and total yield after amplification with barcode primers, these concentrations by ssDNA Qubit quantification seemed to agree to the results below.
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