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==Results== *Even the default E value (10) was used, the reported alignments still have very low (Expect = 1e-31 ~ 9e-33)E-value and high identity (98~100%) of aligned region. It is possible we demand blastn to report only one best output (-K =1) *The is no difference if I change to -e 0.01 or not. Same result came out. *With low-complexity filter turned off (the default is on) I got a little bit more hits. I check the sequence on filtered out hits and found that they should be accurate hits. **Same taxa are found in other non-filtered out hit. **The E-value and Identity rate are high in these filter-out hits.
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