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Date: 10/10/08 '''== DNeasy Experiment==''' Purpose: For purification of total DNA from GC1F fibroblasts cells & PGP1F cells Material & Protocol:[[Media:DNeasy.pdf]] '''Notes''' -2samples: GC1F and PGP1F(red capped 15mL tube) -Broke down cell pellet by pipetting -Without trypsin EDTA, thawed cells from frozen vials. Start from step 2: lysis on own protocol '''Results:''' '''GC1F(Made in Oct.2008)''' gDNA 36.4ng/uL 260/280: 2.01 '''PGP1F (Made in Sep. 2008)''' gDNA: 163.4ng/uL 260/280: 2.08 Both gDNA vials stored in Box 1 in -70freezer located in Kun Lab. Conclusion:With 260/280 ratio close to 2 there seems to be RNA contaminate. In order to obtain RNA-free gDNA prior to step 2 in the protocol 4uL RNase A (100mg/ml) should be added.
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