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14 samples from experiment 1 was sent out for sequencing on Nov 5th, 2007 (sample #3 and 12 were excluded because the reactions were not successful). Since we are particularly interested in sample #14 (rs1264899), two duplicates of this sample were also included (see lab note on Nov-2-07). The sequencing result shows that sample #1,2 (rs3753565), 3 (rs2295680), 12,14,16 (rs 1264899) had multiple peaks on every base pair, which indicates these had bad results. However, SNP locations were successfully found for sample #13 and 15. {| {{table}} | align="center" style="background:#f0f0f0;"|'''Sample''' | align="center" style="background:#f0f0f0;"|'''Sequencing result''' |- | 1 ||no || |- | 2 ||no || |- | 3 ||no || |- | 4 ||yes || |- | 5 ||yes || |- | 6 ||yes || |- | 7 ||yes || |- | 8 ||yes || |- | 9 ||yes || |- | 10 ||yes || |- | 11 ||yes || |- | 12 ||no || |- | 13 ||yes || |- | 14 ||no || |- | 15 ||yes || |- | 16 ||no || |- | |} <b>Sequencing Results:</b><br> The SNP locations for sample #13 and #15 were determined using Genetool and Sequence Scanner, and the result is as follows: <br> SNP: rs1264899<br> Primer: rs1264899_cF/rs1264899_cR <br> Primer location: Hues6Ver2c F1/F4 <br> Sample #13:<br> Template: PGP1 gDNA <br> [[Image:SNP.png]] <br> Sample #15: <br> Template: GC1F cDNA <br> [[Image:SNP2.png]]
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