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Alice:LabNotes/2007-12-12
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<b>Agarose gel on post-PCR product of PGP1Ver3 Primers</b><br> {| {{table}} | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''well 1''' | align="center" style="background:#f0f0f0;"|'''well 2-13''' |- | Low Mass bp Ladder ||3 ul ||0 ul ||0 ul||0 ul||0 ul||0 ul||0 ul||0 ul|| |- | Novex 5x TBE loading dye || 2 ul|| 2 ul|| 2 ul|| 2 ul|| 2 ul|| 2 ul||2 ul||2 ul|| |- | post-PCR sample ||0 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul ||3 ul || |- | |} <br> Note: 20uL SYBEsafe was added to the agrose gel, so no further staining will be needed.<br> [[Image:ZhangLab_2 2007-12-12 13hr 32min.jpg]] <br><b> There was a mistake on this experiment: the reaction was not setup on ice, therefore the reaction would not proceed as expected, whereas the reactions will start at different times. <br> Result: The image above only showed primer dimers, and these bands are NOT the ones we expected. Since the reaction was not setup on ice, therefore they didn't proceed as expected. </b>
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