Editing
Alice:LabNotes/2008-1-11
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
==Experiment 1:Preparation of padlock library== *Real time PCR setup: {| border="1" cellpadding="5" cellspacing="0" |- | align="center" style="background:#f0f0f0;"|''' ''' | align="center" style="background:#f0f0f0;"|'''''' | colspan="5" align="center" style="background:#f0f0f0;"|'''X16''' |- | 2X Taq master mix||50 uL||800uL |- | 100 uM forward primer||0.5uL||8uL |- | 100 uM reverse primer||0.5uL||8uL |- | SYBR Green (50x)||0.5uL||8uL |- | dH2O||48.2||768 |- | Template (10uM)||0.2 uL||3.2uL |- | Total ||100uL ||1600uL |} 94C 2min 20x (94C 30 sec, 58C 2 min, 72C 1 min)<br> 72C 5 min incubate at 15C forever Note: the PCR is usually stopped 3 cycles after the curve goes to plateau. Agrose gel result (1-12-08): [[Image:ZhangLab_2 2008-01-12 15hr 00min.jpeg]] The bright bands on the bottom are the ladders for both columns (3uL ladder, 1 uL TBE buffer, and 1 uL of loading dye), and the rest of the wells contains sample 1-16 (4uL sample and 1uL of loading dye).<br> The bands on the left column (first 8 samples) don't good intensity, which is possibly caused by not well-mixed PCR reagents in the tube.
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information