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==2014 October 09 sort for 1-02 AD and 25-01 Control== * 6 tubes, sorted into 1% BSA ** Hi/Lo PI separation only observed in 1-02 CTX * Spun down at 900 RCF for 10 min * Resuspended each in 100 ul of PBS with 1% BSA and 10% DMSO and counted on BioRad TC20 * Made 10 aliquots each of 10 ul (unless otherwise indicated) and froze down in styrofoam boxes at -80 degC Tubes # 1-02 CTX Full PI, ~25k sorted, aliquoted @ 1.2e5/ml (in 3 ul) # 1-02 CTX Hi PI, ~25k sorted, aliquoted @ 1.2e5/ml (in 3 ul, 7 tubes) # 1-02 CTX Lo PI, ~25k sorted, aliquoted @ 7.83e4/ml # 1-02 CBL Full PI, ~50k sorted, aliquoted @ 2.74e5/ml # 25-01 CTX Full PI, ~13k sorted, aliquoted @ 7.83e4/m (in 20 ul) # 25-01 CBL Full PI, ~50k sorted, aliquoted @ 2.32e5/ml
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